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KITLG ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

KIT ligand

Gene Aliases

C-kit ligand; kit ligand; Mast cell growth factor; mast cell growth factor (white heifer disease) ; MGF; SCF; stem cell factor.

Gene ID

281885

Accession Number

NP_776800.1

Reactivity

Bos taurus, Bovine

Target

Kit ligand

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology KITLG ELISA Kit (Bovine) (OKEH04606) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KITLG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for KITLG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KITLG captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Sensitivity

30 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

31 KDa

Specificity

Natural and recombinant Bovine Kit ligand

Protein Length

274

NCBI Gene Symbol

KITLG

Protein Name

Q28132

Gene Name URL

KITLG

Nucleotide Accession Number

NM_174375.2

Frequently Asked Questions

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