FABP1 ELISA Kit (Bovine)
Product Specifications
Gene Name
Fatty acid binding protein 1
Gene Aliases
Fatty acid binding protein 1, liver; Fatty acid-binding protein 1; fatty acid-binding protein, liver; L-FABP; liver-type fatty acid-binding protein.
Gene ID
327700
Accession Number
NP_787011.1
Reactivity
Bos taurus, Bovine
Target
Fatty acid-binding protein, liver
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FABP1 ELISA Kit (Bovine) (OKEH04603) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FABP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for FABP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FABP1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
6.25-400ng/mL
Sensitivity
3.125 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
14 KDa
Specificity
Natural and recombinant Bovine Fatty acid-binding protein, liver
Protein Length
127
NCBI Gene Symbol
FABP1
Protein Name
P80425
Gene Name URL
FABP1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_175817.3
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