LRP5 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
LDL receptor related protein 5
Gene Aliases
BMND1; EVR1; EVR4; HBM; low density lipoprotein receptor-related protein 5; low density lipoprotein receptor-related protein 7; low-density lipoprotein receptor-related protein 5; LR3; LRP-5; LRP7; LRP-7; OPPG; OPS; OPTA1; PCLD4; VBCH2.
Gene ID
4041
Accession Number
NP_001278831.1
Reactivity
Homo sapiens, Human
Target
Low-density lipoprotein receptor-related protein 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LRP5 ELISA Kit (Human) (OKEH04600) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LRP5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for LRP5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LRP5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.19 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
179 KDa
Specificity
Natural and recombinant Human Low-density lipoprotein receptor-related protein 5
Protein Length
1615
NCBI Gene Symbol
LRP5
Protein Name
O75197
Gene Name URL
LRP5
Nucleotide Accession Number
NM_001291902.1
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