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NR1I3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Nuclear receptor subfamily 1 group I member 3

Gene Aliases

CAR; CAR1; constitutive activator of retinoid response; constitutive active receptor; constitutive active response; constitutive androstane nuclear receptor variant 2; constitutive androstane nuclear receptor variant 3; constitutive androstane nuclear receptor variant 4; constitutive androstane nuclear receptor variant 5; constitutive androstane receptor; MB67; nuclear receptor subfamily 1 group I member 3; orphan nuclear hormone receptor; orphan nuclear receptor MB67.

Gene ID

9970

Accession Number

NP_001070937.1

Reactivity

Homo sapiens, Human

Target

Nuclear receptor subfamily 1 group I member 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NR1I3 ELISA Kit (Human) (OKEH04599) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NR1I3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for NR1I3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NR1I3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.15 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

40 KDa

Specificity

Natural and recombinant Human Nuclear receptor subfamily 1 group I member 3

Protein Length

352

NCBI Gene Symbol

NR1I3

Protein Name

Q14994

Gene Name URL

NR1I3

Nucleotide Accession Number

NM_001077469.2

Frequently Asked Questions

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