NR1I3 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Nuclear receptor subfamily 1 group I member 3
Gene Aliases
CAR; CAR1; constitutive activator of retinoid response; constitutive active receptor; constitutive active response; constitutive androstane nuclear receptor variant 2; constitutive androstane nuclear receptor variant 3; constitutive androstane nuclear receptor variant 4; constitutive androstane nuclear receptor variant 5; constitutive androstane receptor; MB67; nuclear receptor subfamily 1 group I member 3; orphan nuclear hormone receptor; orphan nuclear receptor MB67.
Gene ID
9970
Accession Number
NP_001070937.1
Reactivity
Homo sapiens, Human
Target
Nuclear receptor subfamily 1 group I member 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NR1I3 ELISA Kit (Human) (OKEH04599) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NR1I3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for NR1I3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NR1I3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.15 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
40 KDa
Specificity
Natural and recombinant Human Nuclear receptor subfamily 1 group I member 3
Protein Length
352
NCBI Gene Symbol
NR1I3
Protein Name
Q14994
Gene Name URL
NR1I3
Nucleotide Accession Number
NM_001077469.2
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