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Gdf15 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Growth differentiation factor 15

Gene Aliases

Growth/differentiation factor 15; macrophage inhibiting cytokine-1; macrophage inhibitory cytokine 1; MIC-; MIC-1; NAG; NAG-1; SBF.

Gene ID

23886

Accession Number

NP_035949.2

Reactivity

Mouse, Mus musculus

Target

Growth/differentiation factor 15

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Gdf15 ELISA Kit (Mouse) (OKEH04596) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gdf15 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Gdf15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gdf15 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids

Detection Range

31.2-2000pg/mL

Sensitivity

15.6 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

33 KDa

Specificity

Natural and recombinant Mouse Growth/differentiation factor 15

Protein Length

303

NCBI Gene Symbol

Gdf15

Protein Name

Q9Z0J7

Gene Name URL

Gdf15

Nucleotide Accession Number

NM_011819.2

Frequently Asked Questions

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