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Nrp1 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Neuropilin 1

Gene Aliases

A5 protein; C530029I03; N; neuropilin-1; Neuropilin-1 precursor (A5 protein) ; NP; NP-1; Npn1; NPN-1; Nrp; transmembrane receptor.

Gene ID

18186

Accession Number

NP_032763.2

Reactivity

Mouse, Mus musculus

Target

Neuropilin-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Nrp1 ELISA Kit (Mouse) (OKEH04592) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nrp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Nrp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nrp1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids

Detection Range

78-5000pg/mL

Sensitivity

40.8 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

103 KDa

Specificity

Natural and recombinant Mouse Neuropilin-1

Protein Length

923

NCBI Gene Symbol

Nrp1

Protein Name

P97333

Gene Name URL

Nrp1

Nucleotide Accession Number

NM_008737.2

Frequently Asked Questions

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