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S100a9 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

S100 calcium binding protein A9 (calgranulin B)

Gene Aliases

60B8A;60B8Ag; AW546964; BEE22; Cag; Cagb; Calgranulin-B; GAG; GAGB; L1; L1Ag; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; MRP; MRP14; p1; p14; protein S100-A9; S100 calcium-binding protein A9.

Gene ID

20202

Accession Number

NP_001268781.1

Reactivity

Mouse, Mus musculus

Target

Protein S100-A9

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology S100a9 ELISA Kit (Mouse) (OKEH04588) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100a9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for S100a9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100a9 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

62.5-4000pg/mL

Sensitivity

31.31 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

13 KDa

Specificity

Natural and recombinant Mouse Protein S100-A9

Protein Length

113

NCBI Gene Symbol

S100a9

Protein Name

P31725

Gene Name URL

S100a9

Nucleotide Accession Number

NM_001281852.1

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