S100a9 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
S100 calcium binding protein A9 (calgranulin B)
Gene Aliases
60B8A;60B8Ag; AW546964; BEE22; Cag; Cagb; Calgranulin-B; GAG; GAGB; L1; L1Ag; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; MRP; MRP14; p1; p14; protein S100-A9; S100 calcium-binding protein A9.
Gene ID
20202
Accession Number
NP_001268781.1
Reactivity
Mouse, Mus musculus
Target
Protein S100-A9
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology S100a9 ELISA Kit (Mouse) (OKEH04588) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100a9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for S100a9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100a9 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
62.5-4000pg/mL
Sensitivity
31.31 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
13 KDa
Specificity
Natural and recombinant Mouse Protein S100-A9
Protein Length
113
NCBI Gene Symbol
S100a9
Protein Name
P31725
Gene Name URL
S100a9
Nucleotide Accession Number
NM_001281852.1
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