Mstn ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Myostatin
Gene Aliases
Cmpt; Gdf8; growth/differentiation factor 8; Myostatin.
Gene ID
17700
Accession Number
NP_034964.1
Reactivity
Mouse, Mus musculus
Target
Growth/differentiation factor 8
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Mstn ELISA Kit (Mouse) (OKEH04585) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mstn has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Mstn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mstn captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
78-5000pg/mL
Sensitivity
42.4 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
43 KDa
Specificity
Natural and recombinant Mouse Growth/differentiation factor 8
Protein Length
376
NCBI Gene Symbol
Mstn
Protein Name
O08689
Gene Name URL
Mstn
Nucleotide Accession Number
NM_010834.3
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