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Adgre1 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Adhesion G protein-coupled receptor E1

Gene Aliases

Adhesion G protein-coupled receptor E1; cell surface glycoprotein F4/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly7; Ly71; TM7L; TM7LN3.

Gene ID

13733

Accession Number

NP_034260.1

Reactivity

Mouse, Mus musculus

Target

Adhesion G protein-coupled receptor E1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Adgre1 ELISA Kit (Mouse) (OKEH04581) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adgre1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Adgre1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adgre1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.091 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

102 KDa

Specificity

Natural and recombinant Mouse EGF-like module-containing mucin-like hormone receptor-like 1

Protein Length

931

NCBI Gene Symbol

Adgre1

Protein Name

Q61549

Gene Name URL

Adgre1

Nucleotide Accession Number

NM_010130.4

Frequently Asked Questions

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