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Igfbp5 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Insulin-like growth factor binding protein 5

Gene Aliases

AI256729; AW208790; IBP-5; IGF-binding protein 5; IGFBP; IGFBP-5; IGFBP-5P; insulin-like growth factor-binding protein 5.

Gene ID

16011

Accession Number

NP_034648.2

Reactivity

Mouse, Mus musculus

Target

Insulin-like growth factor-binding protein 5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Igfbp5 ELISA Kit (Mouse) (OKEH04579) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Igfbp5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Igfbp5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Igfbp5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.625-40ng/mL

Sensitivity

0.312 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

30 KDa

Specificity

Natural and recombinant Mouse Insulin-like growth factor-binding protein 5

Protein Length

271

NCBI Gene Symbol

Igfbp5

Protein Name

Q07079

Gene Name URL

Igfbp5

Nucleotide Accession Number

NM_010518.2

Frequently Asked Questions

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