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Th ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Tyrosine hydroxylase

Gene Aliases

The; tyrosine 3-hydroxylase; tyrosine 3-monooxygenase.

Gene ID

25085

Accession Number

NP_036872.1

Reactivity

Rat, Rattus norvegicus

Target

Tyrosine 3-monooxygenase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Th ELISA Kit (Rat) (OKEH04578) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Th has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Th is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Th captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

56 KDa

Specificity

Natural and recombinant Rat Tyrosine 3-monooxygenase

Protein Length

498

NCBI Gene Symbol

Th

Protein Name

P04177

Gene Name URL

Th

Nucleotide Accession Number

NM_012740.3

Frequently Asked Questions

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