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FOS ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Fos proto-oncogene, AP-1 transcription factor subunit

Gene Aliases

Activator protein 1; AP-1; cellular oncogene c-fos; Cellular oncogene fos; C-FOS; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS) ; FBJ murine osteosarcoma viral oncogene homolog; Fos proto-oncogene, AP-1 trancription factor subunit; G0/G1 switch regulatory protein 7; p55; proto-oncogene c-Fos.

Gene ID

2353

Accession Number

NP_005243.1

Reactivity

Homo sapiens, Human

Target

Proto-oncogene c-Fos

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FOS ELISA Kit (Human) (OKEH04574) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FOS has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for FOS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FOS captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.12 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

41 KDa

Specificity

Natural and recombinant Human Proto-oncogene c-Fos

Protein Length

380

NCBI Gene Symbol

FOS

Protein Name

P01100

Gene Name URL

FOS

Nucleotide Accession Number

NM_005252.3

Frequently Asked Questions

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