Tgfb3 ELISA Kit (Rat)
Product Specifications
Gene Name
Transforming growth factor, beta 3
Gene Aliases
TGF-B3; TGF-beta-3; transforming growth factor beta-3 proprotein.
Gene ID
25717
Accession Number
NP_037306.1
Reactivity
Rat, Rattus norvegicus
Target
Transforming growth factor beta-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tgfb3 ELISA Kit (Rat) (OKEH04568) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tgfb3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tgfb3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tgfb3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Sensitivity
8.01 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
47 KDa
Specificity
Natural and recombinant Rat Transforming growth factor beta-3
Protein Length
412
NCBI Gene Symbol
Tgfb3
Protein Name
Q07258
Gene Name URL
Tgfb3
CAS Number
7732-18-5
Nucleotide Accession Number
NM_013174.2
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