Hspa1a ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Heat shock protein family A (Hsp70) member 1A, heat shock protein family A (Hsp70) member 1B
Gene Aliases
Heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock protein 1A; heat shock protein 1B; heat shock protein 70; heat shock protein 70-1; heat shock protein family A (Hsp70) member 1A; heat shock protein family A member 1A; HSP70; HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; Hsp70-1; HSP70-1/HSP70-2; Hsp70-2; HSP72; Hspa1; Hspa1a; Hspa1b; Hspa2.
Gene ID
108348108, 24472, 294254
Accession Number
NP_114177.2
Reactivity
Rat, Rattus norvegicus
Target
Heat shock 70 kDa protein 1A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hspa1a ELISA Kit (Rat) (OKEH04542) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspa1a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Hspa1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hspa1a captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
70 KDa
Specificity
Natural and recombinant Rat Heat shock 70 kDa protein 1A/1B
Protein Length
641
NCBI Gene Symbol
Hspa1a, Hspa1b
Protein Name
P0DMW0
Gene Name URL
Hspa1a, Hspa1b
Nucleotide Accession Number
NM_031971.2
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