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Orm1 ELISA Kit (Rat)

Selected Variant

OKEH04541-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Orosomucoid 1

Gene Aliases

AAG; AGP; Agpa1; alpha-1-acid glycoprotein; OMD; Orm; Orosomucoid.

Gene ID

24614

Accession Number

NP_445740.1

Reactivity

Rat, Rattus norvegicus

Target

Alpha-1-acid glycoprotein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Orm1 ELISA Kit (Rat) (OKEH04541) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Orm1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Orm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Orm1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

7.8-500ng/mL

Sensitivity

3.98 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

24 KDa

Specificity

Natural and recombinant Rat Alpha-1-acid glycoprotein

Protein Length

205

NCBI Gene Symbol

Orm1

Protein Name

P02764

Gene Name URL

Orm1

Nucleotide Accession Number

NM_053288.2

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