PTGDR ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Prostaglandin D receptor
Gene Aliases
D; DP; PGD; PGD receptor; PGD2 receptor; prostaglandin D2 receptor; prostanoid DP receptor.
Gene ID
19214
Accession Number
NP_032988.3
Reactivity
Mouse, Mus musculus
Target
Prostaglandin D2 receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Ptgdr ELISA Kit (Mouse) (OKEH04485) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Ptgdr antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ptgdr and incubated. The found in the sample or standards competes with the biotinylated Ptgdr for limited binding sites on the immobilized anti-Mouse Ptgdr antibody. Excess unbound biotinylated Ptgdr and sample or standard Ptgdr is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ptgdr captured in the well and inversely proportional to the amount of Ptgdr which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
0.318 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
40 KDa
Specificity
Natural and recombinant Mouse Prostaglandin D2 receptor
Protein Length
357
NCBI Gene Symbol
Ptgdr
Protein Name
P70263
Gene Name URL
Ptgdr
Nucleotide Accession Number
NM_008962.4
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