ICAM4 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
Gene Aliases
CD242; CD242 antigen; intercellular adhesion molecule 4; intercellular adhesion molecule 4 (LW blood group) ; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; LW; LW antigen a.
Gene ID
3386
Accession Number
NP_001034221.1
Reactivity
Homo sapiens, Human
Target
Intercellular adhesion molecule 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ICAM4 ELISA Kit (Human) (OKEH04482) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ICAM4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ICAM4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ICAM4 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
29 KDa
Specificity
Natural and recombinant Human Intercellular adhesion molecule 4
Protein Length
271
NCBI Gene Symbol
ICAM4
Protein Name
Q14773
Gene Name URL
ICAM4
Nucleotide Accession Number
NM_001039132.2
Frequently Asked Questions
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