CALR ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Calreticulin
Gene Aliases
CABP3; CALBP; calcium-binding protein 3; calregulin; calreticulin; CRP55; endoplasmic reticulum resident protein 60; ERp60; HACBP.
Gene ID
64202
Accession Number
NP_071794.1
Reactivity
Rat, Rattus norvegicus
Target
Calreticulin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Calr ELISA Kit (Rat) (OKEH04474) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Calr has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Calr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Calr captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.39-25ng/mL
Sensitivity
0.121 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
48 KDa
Specificity
Natural and recombinant Rat Calreticulin
Protein Length
416
NCBI Gene Symbol
Calr
Protein Name
P18418
Gene Name URL
Calr
Nucleotide Accession Number
NM_022399.2
Frequently Asked Questions
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HLA-DRA (DR alpha Chain, DR alpha Chain Precursor, DRA, DRB1, DRB4, HLA class II histocompatibility Antigen, HLA class II histocompatibility Antigen DR alpha Chain, HLA DR1B, HLA DR3B, HLA DRA, HLA DRA1, HLA DRB1, HLA DRB3, HLA DRB4, HLA DRB5, HLA-DRA, HLADR4B, HLADRA1, HLADRB, Major histocompatibility Complex class II DR alpha, Major histocompatibility Complex class II DR beta 1, Major histocompatibility Complex class II DR beta 3, Major histocompatibility Complex class II DR beta 4, Major histoco) discontinued
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