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LAMC2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Laminin subunit gamma 2

Gene Aliases

B2T; BM600; BM600-100kDa; cell-scattering factor 140 kDa subunit; CSF; CSF 140 kDa subunit; EBR2; EBR2A; epiligrin subunit gamma; kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; ladsin 140 kDa subunit; LAMB2T; laminin B2t chain; laminin subunit gamma-2; laminin, gamma 2; Laminin-5 subunit gamma; LAMNB2; large adhesive scatter factor 140 kDa subunit; nicein subunit gamma.

Gene ID

3918

Accession Number

NP_005553.2

Reactivity

Homo sapiens, Human

Target

Laminin subunit gamma-2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LAMC2 ELISA Kit (Human) (OKEH04464) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAMC2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for LAMC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAMC2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

21 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

131 KDa

Specificity

Natural and recombinant Human Laminin subunit gamma-2

Protein Length

1193

NCBI Gene Symbol

LAMC2

Protein Name

Q13753

Gene Name URL

LAMC2

Nucleotide Accession Number

NM_005562.2

Frequently Asked Questions

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