ARG1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Arginase 1
Gene Aliases
AI type I arginase; arginase, liver; arginase-1; liver-type arginase; type I arginase.
Gene ID
29221
Accession Number
NP_058830.2
Reactivity
Rat, Rattus norvegicus
Target
Arginase-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Arg1 ELISA Kit (Rat) (OKEH04455) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Arg1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Arg1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Arg1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
2.5-160ng/mL
Sensitivity
1.25 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
35 KDa
Specificity
Natural and recombinant Rat Arginase-1
Protein Length
323
NCBI Gene Symbol
Arg1
Protein Name
P07824
Gene Name URL
Arg1
Nucleotide Accession Number
NM_017134.3
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