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EPHA3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

EPH receptor A3

Gene Aliases

EK4; EPH-like kinase 4; eph-like tyrosine kinase 1; ephrin type-A receptor 3; ETK; ETK1; HEK; HEK4; human embryo kinase 1; testicular tissue protein Li 64; TYRO4; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1; Tyrosine-protein kinase TYRO4.

Gene ID

2042

Accession Number

NP_005224.2

Reactivity

Homo sapiens, Human

Target

Ephrin type-A receptor 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology EPHA3 ELISA Kit (Human) (OKEH04453) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPHA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for EPHA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EPHA3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%

Sensitivity

0.11 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

110 KDa

Specificity

Natural and recombinant Human Ephrin type-A receptor 3

Protein Length

983

NCBI Gene Symbol

EPHA3

Protein Name

P29320

Gene Name URL

EPHA3

Nucleotide Accession Number

NM_005233.5

Frequently Asked Questions

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