MYLK ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Myosin light chain kinase
Gene Aliases
AAT7; kinase-related protein; KRP; MLCK; MLCK1; MLCK108; MLCK210; MMIHS; MMIHS1; MSTP083; MYLK1; myosin light chain kinase, smooth muscle; myosin, light polypeptide kinase; smMLCK; smooth muscle myosin light chain kinase; telokin.
Gene ID
4638
Accession Number
NP_001308238.1
Reactivity
Homo sapiens, Human
Target
Myosin light chain kinase, smooth muscle
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MYLK ELISA Kit (Human) (OKEH04450) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYLK has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for MYLK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYLK captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
0.076 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
211 KDa
Specificity
Natural and recombinant Human Myosin light chain kinase, smooth muscle
Protein Length
1914
NCBI Gene Symbol
MYLK
Protein Name
Q15746
Gene Name URL
MYLK
Nucleotide Accession Number
NM_001321309.1
Frequently Asked Questions
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