ACTN3 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
Actinin alpha 3
Gene Aliases
ACTN3D; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 3; alpha-actinin-3; F-actin cross-linking protein.
Gene ID
89
Accession Number
NP_001095.2
Reactivity
Homo sapiens, Human
Target
Alpha-actinin-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ACTN3 ELISA Kit (Human) (OKEH04449) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACTN3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ACTN3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACTN3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
7.8-500pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
4.1 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
103 KDa
Specificity
Natural and recombinant Human Alpha-actinin-3
Protein Length
901
NCBI Gene Symbol
ACTN3
Protein Name
Q08043
Gene Name URL
ACTN3
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001104.3
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