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ADAD1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Adenosine deaminase domain containing 1

Gene Aliases

Adenosine deaminase domain containing 1 (testis-specific) ; adenosine deaminase domain-containing protein 1; Tenr; testis nuclear RNA-binding protein; testis tissue sperm-binding protein Li 92mP.

Gene ID

132612

Accession Number

NP_001152757.1

Reactivity

Homo sapiens, Human

Target

Adenosine deaminase domain-containing protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ADAD1 ELISA Kit (Human) (OKEH04447) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAD1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ADAD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAD1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.11 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

64 KDa

Specificity

Natural and recombinant Human Adenosine deaminase domain-containing protein 1

Protein Length

576

NCBI Gene Symbol

ADAD1

Protein Name

Q96M93

Gene Name URL

ADAD1

Nucleotide Accession Number

NM_001159285.1

Frequently Asked Questions

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