ATP6AP2 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
ATPase H+ transporting accessory protein 2
Gene Aliases
APT6M8-9; ATP6IP2; ATP6M8-9; ATPase H (+) -transporting lysosomal accessory protein 2; ATPase H (+) -transporting lysosomal-interacting protein 2; ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9; ATPase, H+ transporting, lysosomal accessory protein 2; ATPase, H+ transporting, lysosomal interacting protein 2; CDG2R; ELDF10; embryonic liver differentiation factor 10; ER-localized type I transmembrane adapter; ER-localized type I transmembrane adaptor; HT028; M8-9; MRXE; MRXSH; MSTP009; N14F; prorenin receptor; PRR; renin receptor; renin/prorenin receptor; RENR; vacuolar ATP synthase membrane sector-associated protein M8-9; vacuolar proton ATP synthase membrane sector associated protein M8-9; V-ATPase M8.9 subunit; XMRE; XPDS.
Gene ID
10159
Accession Number
NP_005756.2
Reactivity
Homo sapiens, Human
Target
Renin receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ATP6AP2 ELISA Kit (Human) (OKEH04445) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP6AP2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ATP6AP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP6AP2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 KDa
Specificity
Natural and recombinant Human Renin receptor
Protein Length
350
NCBI Gene Symbol
ATP6AP2
Protein Name
O75787
Gene Name URL
ATP6AP2
Nucleotide Accession Number
NM_005765.2
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