CYP7A1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Cytochrome P450 family 7 subfamily A member 1
Gene Aliases
24-hydroxycholesterol 7-alpha-hydroxylase; CHAP; cholesterol 7-alpha-hydroxylase; cholesterol 7-alpha-monooxygenase; CYP7; CYP7S1; CYPVII; cytochrome P450 (cholesterol hydroxylase 7 alpha) ; cytochrome P450 7A1; cytochrome P450, 7a1; cytochrome P450, family 7, subfamily a, polypeptide 1.
Gene ID
25428
Accession Number
NP_037074.1
Reactivity
Rat, Rattus norvegicus
Target
Cholesterol 7-alpha-monooxygenase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cyp7a1 ELISA Kit (Rat) (OKEH04424) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp7a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Cyp7a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp7a1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
7.8-500pg/mL
Sensitivity
3.95 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 KDa
Specificity
Natural and recombinant Rat Cholesterol 7-alpha-monooxygenase
Protein Length
503
NCBI Gene Symbol
Cyp7a1
Protein Name
P18125
Gene Name URL
Cyp7a1
Nucleotide Accession Number
NM_012942.2
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