ZNF335 ELISA Kit (Mouse)
Selected Variant
OKEH04412-96W
Size: 96 Well
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Zinc finger protein 335
Gene Aliases
1810045J01Rik; Nif1; NIF-1; NRC-interacting factor 1; zinc finger protein 335; Znf335.
Gene ID
329559
Accession Number
NP_950192.2
Reactivity
Mouse, Mus musculus
Target
Zinc finger protein 335
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Znf335 ELISA Kit (Mouse) (OKEH04412) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Znf335 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Znf335 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Znf335 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.158 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
146 KDa
Specificity
Natural and recombinant Mouse Zinc finger protein 335
Protein Length
1337
NCBI Gene Symbol
Zfp335
Protein Name
A2A5K6
Gene Name URL
Zfp335
Nucleotide Accession Number
NM_199027.2
Price
Sign In for Pricing
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog