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VLDLR ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Very low density lipoprotein receptor

Gene Aliases

Hlb301, LDL receptor, Low-density lipoprotein receptor

Gene ID

25696

Accession Number

NP_037287.2

Reactivity

Rat, Rattus norvegicus

Target

Very low-density lipoprotein receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Vldlr ELISA Kit (Rat) (OKEH04407) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vldlr has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Vldlr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Vldlr captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

1.56-100ng/mL

Sensitivity

0.78 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

97 KDa

Specificity

Natural and recombinant Rat Very low-density lipoprotein receptor

Protein Length

873

NCBI Gene Symbol

VLDLR

Protein Name

P98166

Gene Name URL

VLDLR

Nucleotide Accession Number

NM_013155.2

Frequently Asked Questions

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