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PMP2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Peripheral myelin protein 2

Gene Aliases

Myelin P2 protein; P; P2.

Gene ID

18857

Accession Number

NP_001025476.1

Reactivity

Mouse, Mus musculus

Target

Myelin P2 protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Pmp2 ELISA Kit (Mouse) (OKEH04401) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pmp2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Pmp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pmp2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%

Sensitivity

0.157 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

15 KDa

Specificity

Natural and recombinant Mouse Myelin P2 protein

Protein Length

132

NCBI Gene Symbol

Pmp2

Protein Name

P24526

Gene Name URL

Pmp2

Nucleotide Accession Number

NM_001030305.2

Frequently Asked Questions

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