PPIA ELISA Kit (Rat)
Product Specifications
Gene Name
Peptidylprolyl isomerase A, peptidylprolyl isomerase A (cyclophilin A) -like 4D
Gene Aliases
CYCA; cyclophilin A; cyclosporin A-binding protein; CyP-A; p1B15; p31; peptidyl-prolyl cis-trans isomerase A; PPIase A; rotamase A; TRIMCyp-like.
Gene ID
100360977, 25518
Accession Number
NP_058797.1
Reactivity
Rat, Rattus norvegicus
Target
Peptidyl-prolyl cis-trans isomerase A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ppia ELISA Kit (Rat) (OKEH04397) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppia has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Ppia is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppia captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
18 KDa
Specificity
Natural and recombinant Rat Peptidyl-prolyl cis-trans isomerase A
Protein Length
164
NCBI Gene Symbol
Ppia, Ppial4d
Protein Name
P10111
Gene Name URL
Ppia, Ppial4d
CAS Number
7732-18-5
Nucleotide Accession Number
NM_017101.1
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