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PPIA ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Peptidylprolyl isomerase A

Gene Aliases

Cp; Cphn; Cy; cyclophilin A; cyclosporin A-binding protein; CyP-1; CyP-18; CypA; peptidyl-prolyl cis-trans isomerase A; PPIase A; rotamase A; SP18.

Gene ID

268373

Accession Number

NP_032933.1

Reactivity

Mouse, Mus musculus

Target

Peptidyl-prolyl cis-trans isomerase A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ppia ELISA Kit (Mouse) (OKEH04396) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppia has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Ppia is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppia captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%

Sensitivity

0.403 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

18 KDa

Specificity

Natural and recombinant Mouse Peptidyl-prolyl cis-trans isomerase A

Protein Length

164

NCBI Gene Symbol

Ppia

Protein Name

P17742

Gene Name URL

Ppia

Nucleotide Accession Number

NM_008907.1

Frequently Asked Questions

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