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ABCG2 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

ATP binding cassette subfamily G member 2 (Junior blood group)

Gene Aliases

ABC15; ABCP; AI428558; ATP-binding cassette sub-family G member 2; ATP-binding cassette, sub-family G (WHITE), member 2; Bc; BCRP; Bcrp1; breast cancer resistance protein 1 homolog; broad substrate specificity ATP-binding cassette transporter ABCG2; mitoxantrone resistance protein 1; MXR; MXR1; urate exporter.

Gene ID

26357

Accession Number

NP_036050.1

Reactivity

Mouse, Mus musculus

Target

ATP-binding cassette sub-family G member 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology Abcg2 ELISA Kit (Mouse) (OKEH04395) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Abcg2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Abcg2 and incubated. The found in the sample or standards competes with the biotinylated Abcg2 for limited binding sites on the immobilized anti-Mouse Abcg2 antibody. Excess unbound biotinylated Abcg2 and sample or standard Abcg2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Abcg2 captured in the well and inversely proportional to the amount of Abcg2 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%

Sensitivity

0.165 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

73 KDa

Specificity

Natural and recombinant Mouse ATP-binding cassette sub-family G member 2

Protein Length

657

NCBI Gene Symbol

Abcg2

Protein Name

Q7TMS5

Gene Name URL

Abcg2

Nucleotide Accession Number

NM_011920.3

Frequently Asked Questions

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