What is the catalog number for PCK1 ELISA Kit (Human)?

The catalog number for PCK1 ELISA Kit (Human) is OKEH04391-96W.

What sizes are available for PCK1 ELISA Kit (Human)?

PCK1 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is PCK1 ELISA Kit (Human) in stock?

PCK1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

PCK1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Phosphoenolpyruvate carboxykinase 1

Gene Aliases

PCKDC; PEP carboxykinase; PEPCK1; PEPCKC; PEPCK-C; phosphoenolpyruvate carboxykinase 1 (soluble) ; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; serine-protein kinase PCK1.

Gene ID

5105

Accession Number

NP_002582.3

Reactivity

Homo sapiens, Human

Target

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PCK1 ELISA Kit (Human) (OKEH04391) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PCK1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for PCK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PCK1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol