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DNAJB1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

DnaJ heat shock protein family (Hsp40) member B1

Gene Aliases

DnaJ (Hsp40) homolog, subfamily B, member 1; dnaJ homolog subfamily B member 1; dnaJ protein homolog 1; Hdj1; heat shock 40 kDa protein 1; Hsp40; HSPF1; human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1.

Gene ID

3337

Accession Number

NP_001287843.1

Reactivity

Homo sapiens, Human

Target

DnaJ homolog subfamily B member 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DNAJB1 ELISA Kit (Human) (OKEH04386) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNAJB1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for DNAJB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNAJB1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

93.75-6000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

Sensitivity

45 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

38 KDa

Specificity

Natural and recombinant Human DnaJ homolog subfamily B member 1

Protein Length

340

NCBI Gene Symbol

DNAJB1

Protein Name

P25685

Gene Name URL

DNAJB1

Nucleotide Accession Number

NM_001300914.1

Frequently Asked Questions

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