What is the catalog number for SPARC ELISA Kit (Mouse)?

The catalog number for SPARC ELISA Kit (Mouse) is OKEH04375-96W.

What sizes are available for SPARC ELISA Kit (Mouse)?

SPARC ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is SPARC ELISA Kit (Mouse) in stock?

SPARC ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

SPARC ELISA Kit (Mouse)

Selected Variant

OKEH04375-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Secreted acidic cysteine rich glycoprotein

Gene Aliases

Basement-membrane protein 40; BM-40; ON; osteo; osteonectin; secreted protein acidic and rich in cysteine; SPARC.

Gene ID

20692

Accession Number

NP_033268.1

Reactivity

Mouse, Mus musculus

Target

SPARC

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Sparc ELISA Kit (Mouse) (OKEH04375) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sparc has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Sparc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sparc captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

3.12-200ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116%

Sensitivity

1.68 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

34 KDa

Specificity

Natural and recombinant Mouse SPARC

Protein Length

302

NCBI Gene Symbol

Sparc

Protein Name

P07214

Gene Name URL

Sparc

Nucleotide Accession Number

NM_009242.5

Price

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