IMPA2 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Inositol monophosphatase 2
Gene Aliases
IMP 2; IMPase 2; inosine monophosphatase 2; inositol monophosphatase 2; inositol monophosphatase 2 variant 1; inositol monophosphatase 2 variant 2; inositol (myo) -1 (or 4) -monophosphatase 2; Inositol-1 (or 4) -monophosphatase 2; myo-inositol monophosphatase 2; myo-inositol monophosphatase A2.
Gene ID
3613
Accession Number
NP_055029.1
Reactivity
Homo sapiens, Human
Target
Inositol monophosphatase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IMPA2 ELISA Kit (Human) (OKEH04359) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IMPA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for IMPA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IMPA2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
62.5-4000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
37 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 KDa
Specificity
Natural and recombinant Human Inositol monophosphatase 2
Protein Length
288
NCBI Gene Symbol
IMPA2
Protein Name
O14732
Gene Name URL
IMPA2
Nucleotide Accession Number
NM_014214.2
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