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IMPA2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Inositol monophosphatase 2

Gene Aliases

IMP 2; IMPase 2; inosine monophosphatase 2; inositol monophosphatase 2; inositol monophosphatase 2 variant 1; inositol monophosphatase 2 variant 2; inositol (myo) -1 (or 4) -monophosphatase 2; Inositol-1 (or 4) -monophosphatase 2; myo-inositol monophosphatase 2; myo-inositol monophosphatase A2.

Gene ID

3613

Accession Number

NP_055029.1

Reactivity

Homo sapiens, Human

Target

Inositol monophosphatase 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology IMPA2 ELISA Kit (Human) (OKEH04359) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IMPA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for IMPA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IMPA2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

37 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

31 KDa

Specificity

Natural and recombinant Human Inositol monophosphatase 2

Protein Length

288

NCBI Gene Symbol

IMPA2

Protein Name

O14732

Gene Name URL

IMPA2

Nucleotide Accession Number

NM_014214.2

Frequently Asked Questions

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