AKR1C6 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Aldo-keto reductase family 1, member C6
Gene Aliases
17-beta-HSD 5;17HSD type 5;3alph;3alpha-HSD; Akr1; Akr1c1; aldo-keto reductase family 1, member C1 (aldehyde reductase) ; estradiol 17 beta-dehydrogenase 5; estradiol 17-beta-dehydrogenase (A-specific) ; Hsd17b; Hsd17b5; hydroxysteroid 17-beta dehydrogenase 5.
Gene ID
83702
Accession Number
NP_085114.1
Reactivity
Mouse, Mus musculus
Target
Estradiol 17 beta-dehydrogenase 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Akr1c6 ELISA Kit (Mouse) (OKEH04358) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Akr1c6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Akr1c6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Akr1c6 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 KDa
Specificity
Natural and recombinant Mouse Estradiol 17 beta-dehydrogenase 5
Protein Length
323
NCBI Gene Symbol
Akr1c6
Protein Name
P70694
Gene Name URL
Akr1c6
Nucleotide Accession Number
NM_030611.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
bs-10075R-BF680 100 µL
Nidogen2 Polyclonal Antibody, AbBy Fluor™ 680 Conjugated
Sign In for Pricing
View Details