MMP24 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Matrix metallopeptidase 24
Gene Aliases
AU040325; matrix metalloproteinase-21; matrix metalloproteinase-24; membrane type 5-MMP; membrane-type matrix metalloproteinase 5; Membrane-type-5 matrix metalloproteinase; MMP-21; MT5-MM; MT5MMP; MT5-MMP; MTMMP5.
Gene ID
17391
Accession Number
NP_034938.3
Reactivity
Mouse, Mus musculus
Target
Matrix metalloproteinase-24
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Mmp24 ELISA Kit (Mouse) (OKEH04346) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mmp24 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mmp24 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mmp24 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
15.71 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
70 KDa
Specificity
Natural and recombinant Mouse Matrix metalloproteinase-24
Protein Length
618
NCBI Gene Symbol
Mmp24
Protein Name
Q9R0S2
Gene Name URL
Mmp24
Nucleotide Accession Number
NM_010808.4
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
