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MMP24 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Matrix metallopeptidase 24

Gene Aliases

AU040325; matrix metalloproteinase-21; matrix metalloproteinase-24; membrane type 5-MMP; membrane-type matrix metalloproteinase 5; Membrane-type-5 matrix metalloproteinase; MMP-21; MT5-MM; MT5MMP; MT5-MMP; MTMMP5.

Gene ID

17391

Accession Number

NP_034938.3

Reactivity

Mouse, Mus musculus

Target

Matrix metalloproteinase-24

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Mmp24 ELISA Kit (Mouse) (OKEH04346) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mmp24 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mmp24 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mmp24 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%

Sensitivity

15.71 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

70 KDa

Specificity

Natural and recombinant Mouse Matrix metalloproteinase-24

Protein Length

618

NCBI Gene Symbol

Mmp24

Protein Name

Q9R0S2

Gene Name URL

Mmp24

Nucleotide Accession Number

NM_010808.4

Frequently Asked Questions

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