Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

GDF5 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Growth differentiation factor 5

Gene Aliases

B; BMP-14; bone morphogenetic protein 14; bp; brp; cartilage-derived morphogenetic protein-1; CDMP-; Cdmp-1; growth/differentiation factor 5.

Gene ID

14563

Accession Number

NP_032135.2

Reactivity

Mouse, Mus musculus

Target

Growth/differentiation factor 5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Gdf5 ELISA Kit (Mouse) (OKEH04345) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gdf5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Gdf5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gdf5 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%

Sensitivity

15.7 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

55 KDa

Specificity

Natural and recombinant Mouse Growth/differentiation factor 5

Protein Length

495

NCBI Gene Symbol

Gdf5

Protein Name

P43027

Gene Name URL

Gdf5

Nucleotide Accession Number

NM_008109.2

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

SIT (F-9) Alexa Fluor® 488
sc-271933 AF488 200 µg/mL

SIT (F-9) Alexa Fluor® 488

Sign In for Pricing
View Details
GPC1 Antibody
MBS7052009-01 0.05 mg

GPC1 Antibody

Sign In for Pricing
View Details
GPC1 Antibody
MBS7052009-02 0.1 mg

GPC1 Antibody

Sign In for Pricing
View Details
GPC1 Antibody
MBS7052009-03 5x 0.1 mg

GPC1 Antibody

Sign In for Pricing
View Details
Mouse Monoclonal HAND1 Antibody (OTI1G10) [Biotin]
NBP2-71459B 0.1 mL

Mouse Monoclonal HAND1 Antibody (OTI1G10) [Biotin]

Sign In for Pricing
View Details
Pigeon Poly ADP Ribose Polymerase 2 (PARP2) ELISA Kit
MBS9343458 Inquire

Pigeon Poly ADP Ribose Polymerase 2 (PARP2) ELISA Kit

Sign In for Pricing
View Details