LMAN2 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Lectin, mannose binding 2
Gene Aliases
C5orf8; epididymis secretory sperm binding protein; glycoprotein GP36b; GP36B; Lectin mannose-binding 2; vesicular integral protein of 36 kDa; vesicular integral-membrane protein 36; vesicular integral-membrane protein VIP36; VIP36.
Gene ID
10960
Accession Number
NP_006807.1
Reactivity
Homo sapiens, Human
Target
Vesicular integral-membrane protein VIP36
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LMAN2 ELISA Kit (Human) (OKEH04344) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LMAN2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for LMAN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LMAN2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
40 KDa
Specificity
Natural and recombinant Human Vesicular integral-membrane protein VIP36
Protein Length
356
NCBI Gene Symbol
LMAN2
Protein Name
Q12907
Gene Name URL
LMAN2
Nucleotide Accession Number
NM_006816.2
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