LAP3 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Leucine aminopeptidase 3
Gene Aliases
Cysteinylglycine-S-conjugate dipeptidase; cytosol aminopeptidase; epididymis secretory protein Li 106; HEL-S-106; LAP; LAPEP; Leucine aminopeptidase 3; leucyl aminopeptidase; PEPS; peptidase S; proline aminopeptidase; prolyl aminopeptidase.
Gene ID
51056
Accession Number
NP_056991.2
Reactivity
Homo sapiens, Human
Target
Cytosol aminopeptidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LAP3 ELISA Kit (Human) (OKEH04342) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAP3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Recovery
< 6.2%
Sensitivity
0.082 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
56 kDa
Specificity
Natural and recombinant Human Cytosol aminopeptidase
Protein Length
519
NCBI Gene Symbol
LAP3
Protein Name
P28838
Gene Name URL
LAP3
Nucleotide Accession Number
NM_015907.2
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