What is the catalog number for HIF1AN ELISA Kit (Human)?

The catalog number for HIF1AN ELISA Kit (Human) is OKEH04329-96W.

What sizes are available for HIF1AN ELISA Kit (Human)?

HIF1AN ELISA Kit (Human) is available in the following size(s): 96 Well.

Is HIF1AN ELISA Kit (Human) in stock?

HIF1AN ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

HIF1AN ELISA Kit (Human)

Selected Variant

OKEH04329-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Hypoxia inducible factor 1 subunit alpha inhibitor

Gene Aliases

Factor inhibiting HIF1; factor inhibiting HIF-1; FIH1; FIH-1; hypoxia inducible factor 1 alpha subunit inhibitor; hypoxia-inducible factor 1-alpha inhibitor; hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase.

Gene ID

55662

Accession Number

NP_060372.2

Reactivity

Homo sapiens, Human

Target

Hypoxia-inducible factor 1-alpha inhibitor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HIF1AN ELISA Kit (Human) (OKEH04329) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIF1AN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIF1AN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HIF1AN captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

0.12 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

40 kDa

Specificity

Natural and recombinant Human Hypoxia-inducible factor 1-alpha inhibitor

Protein Length

349

NCBI Gene Symbol

HIF1AN

Protein Name

Q9NWT6

Gene Name URL

HIF1AN

Nucleotide Accession Number

NM_017902.2

Price

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

CD2 / Lymphocyte Function Antigen 2 (LFA-2) (UMCD2), CF640R conjugate, 0.1mg/mL

BNC400201-500 1x 500 µL