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NAA20 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

N-alpha-acetyltransferase 20, NatB catalytic subunit

Gene Aliases

DJ1002M8.1; methionine N-acetyltransferase; N-acetyltransferase 3 homolog; N-acetyltransferase 5; N-acetyltransferase 5 (ARD1 homolog, S. cerevisiae) ; N-acetyltransferase 5 (GCN5-related, putative) ; N-acetyltransferase 5, ARD1 subunit (arrest-defective 1, S. cerevisiae, homolog) ; N-alpha-acetyltransferase 20; NAT3; NAT3P; NAT5; NAT5P; natB catalytic subunit; natB complex subunit NAT5; N-terminal acetyltransferase B complex catalytic subunit NAA20; N-terminal acetyltransferase B complex catalytic subunit NAT5; N-terminal acetyltransferase complex ARD1 subunit.

Gene ID

51126

Accession Number

NP_057184.1

Reactivity

Homo sapiens, Human

Target

N-alpha-acetyltransferase 20

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NAA20 ELISA Kit (Human) (OKEH04328) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NAA20 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NAA20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NAA20 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%

Sensitivity

0.98 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

20 kDa

Specificity

Natural and recombinant Human N-alpha-acetyltransferase 20

Protein Length

178

NCBI Gene Symbol

NAA20

Protein Name

P61599

Gene Name URL

NAA20

Nucleotide Accession Number

NM_016100.4

Frequently Asked Questions

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