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SFRP2 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Secreted frizzled related protein 2

Gene Aliases

FRP-2; SARP1; SARP-1; SDF-5; secreted apoptosis related protein 1; Secreted apoptosis-related protein 1; secreted frizzled-related protein 2; sFRP-2; testicular tissue protein Li 170.

Gene ID

6423

Accession Number

NP_003004.1

Reactivity

Homo sapiens, Human

Target

Secreted frizzled-related protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SFRP2 ELISA Kit (Human) (OKEH04322) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFRP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFRP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFRP2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

1.25-80ng/mL

Recovery

<= 5.9%

Sensitivity

0.65 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

33 kDa

Specificity

Natural and recombinant Human Secreted frizzled-related protein 2

Protein Length

295

NCBI Gene Symbol

SFRP2

Protein Name

Q96HF1

Gene Name URL

SFRP2

Nucleotide Accession Number

NM_003013.2

Frequently Asked Questions

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