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LOXL3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Lysyl oxidase like 3

Gene Aliases

LOXL; lysyl oxidase homolog 3; lysyl oxidase-like protein 3.

Gene ID

84695

Accession Number

NP_001276093.1

Reactivity

Homo sapiens, Human

Target

Lysyl oxidase homolog 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LOXL3 ELISA Kit (Human) (OKEH04319) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LOXL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LOXL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LOXL3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.47-30nmol/L

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.22nmol/L

Reconstitution

Store as indicated in product manual.

Molecular Weight

83 kDa

Specificity

Natural and recombinant Human Lysyl oxidase homolog 3

Protein Length

753

NCBI Gene Symbol

LOXL3

Protein Name

P58215

Gene Name URL

LOXL3

Nucleotide Accession Number

NM_001289164.1

Frequently Asked Questions

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