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JAK3 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Janus kinase 3

Gene Aliases

JAK-3; Janus kinase 3; Janus kinase 3, protein-tyrosine kinase; RATJAK3; tyrosine-protein kinase JAK3.

Gene ID

25326

Accession Number

NP_036987.2

Reactivity

Rat, Rattus norvegicus

Target

Tyrosine-protein kinase JAK3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Jak3 ELISA Kit (Rat) (OKEH04318) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Jak3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Jak3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Jak3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

0.78-50ng/mL

Sensitivity

0.426 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

123 kDa

Specificity

Natural and recombinant Rat Tyrosine-protein kinase JAK3

Protein Length

1100

NCBI Gene Symbol

Jak3

Protein Name

Q63272

Gene Name URL

Jak3

Nucleotide Accession Number

NM_012855.2

Frequently Asked Questions

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