FUT6 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Fucosyltransferase 6
Gene Aliases
4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase FUT6; alpha- (1,3) -fucosyltransferase 6; FCT3A; FT1A; Fucosyltransferase 6; fucosyltransferase 6 (alpha (1,3) fucosyltransferase) ; fucosyltransferase VI; FucT-VI; Fuc-TVI; galactoside 3-L-fucosyltransferase.
Gene ID
2528
Accession Number
NP_000141.1
Reactivity
Homo sapiens, Human
Target
Alpha- (1,3) -fucosyltransferase 6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FUT6 ELISA Kit (Human) (OKEH04307) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FUT6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FUT6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FUT6 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.057 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Human Alpha- (1,3) -fucosyltransferase 6
Protein Length
359
NCBI Gene Symbol
FUT6
Protein Name
P51993
Gene Name URL
FUT6
Nucleotide Accession Number
NM_000150.2
Frequently Asked Questions
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