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NCOR1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Nuclear receptor corepressor 1

Gene Aliases

HN-CoR; N-CoR; N-CoR1; nuclear receptor corepressor 1; PPP1R109; protein phosphatase 1, regulatory subunit 109; thyroid hormone- and retinoic acid receptor-associated corepressor 1; TRAC1.

Gene ID

9611

Accession Number

NP_001177367.1

Reactivity

Homo sapiens, Human

Target

Nuclear receptor corepressor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NCOR1 ELISA Kit (Human) (OKEH04303) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NCOR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NCOR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NCOR1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

270 kDa

Specificity

Natural and recombinant Human Nuclear receptor corepressor 1

Protein Length

2440

NCBI Gene Symbol

NCOR1

Protein Name

O75376

Gene Name URL

NCOR1

Nucleotide Accession Number

NM_001190438.1

Frequently Asked Questions

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